K-PAM: Klebsiella species serotype predictor and surface antigens modeler

... An In silico Diagnostic Tool



K antigens or capsular polysaccharides (CPS) are repeating units of sugars linked by glycosidic linkages. The sugar composition and the linkages have been determined by chemical, enzymatic methods so far, some of which include methylation analysis, bacteriophage borne endo-glycanases, and Smith degradation analysis. These methods have been further supported by 1H- and 13C-NMR. (Nuclear magnetic resonance) spectra, ORD (Optical rotatory dispersion) and CD (Circular dichroism) spectra of the oligosaccharides.The database comprises three dimensional structures of the K antigens, that were modeled in GLYCAM web server (Woods Group. (2005-2015) GLYCAM Web. Complex Carbohydrate Research Center, University of Georgia, Athens, GA. (http://www.glycam.com)) and were subsequently minimized by CHARMM 36 forcefield. The K antigens are grouped according to their reference species information (Pan et al., 2015).

Click on the links below to get 3D structural information on the antigen
Alll schematic representations of CPS repeating units are grouped majorly into two categories: (i) glucose as first monomer and (ii) galactose as first monomer.




K. pneumoniae
Subspecies: pneumoniae
KK1 KK2 KK7 KK8 KK9 KK10 KK11 KK12 KK13 KK14
KK15 KK16 KK17 KK18 KK19 KK20 KK21 KK22 KK23 KK24
KK25 KK27 KK28 KK30 KK31 KK33 KK34 KK36 KK37 KK38
KK39 KK40 KK43 KK45 KK46 KK47 KK50 KK51 KK55 KK60
KK61 KK62 KK63 KK64 KK80 KK81 KK82
Subspecies: rhinoscleromatis
KK3
Subspecies: ozaenae
KK4 KK5 KK6

K. michiganensis
KK41 KK59 KK66 KK70

K. oxytoca
KK26 KK74

K. variicola
KK48 KK49 KK53 KK54 KK56 KK57 KK58 KK71

K.(Raoultella) planticola
KK35 KK79

K.(Raoultella) ornithinolytica
KK32 KK44 KK72

K.(Raoultella) terrigena
KK65 KK67 KK68 KK69

Others
KK73 KK83